Top 10: Biotechnology

1. Polymerase Chain Reaction (PCR) requires the polymerase called Taq polymerase.  This polymerase comes from hot springs.

2. It is not until after the 3rd crycle that the desired segment of DNA is replicated solely on its own.

3. Sanger's Sequencing requires dideoxyribose nucleotides (ddNTPs)

4. The bacterial plasmid is used to insert a foreign gene as it reproduces very quickly, is easy to obtain, and because we know a lot about the plasmid and bacteria.

5.Restriction enzymes cut the phosphodiester bonds on both strands of DNA in either sticky ends or blunt ends.

6. PCR has three steps: heating, cooling, and replication

7. Southern blotting is used to transfer DNA fragments from the gel to a sheet of nitrocellulose paper.

8. Gel electrophoresis separates macromolecules

9. RFLP markers serve as genetic markers

10. Recombinant DNA is when two genes from different sources are combined.